Expert Writing (1997): Studying the Secondary Structure of Ribosomal RNA Using Atomic Force Microscopy

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Abstract: Ribosomal RNA (rRNA) was isolated using a repeated freeze-thaw method, which effectively disrupted ribosomes at low temperatures. The rRNA remained intact and could be well dispersed on mica surfaces without the need for extraction, denaturation, or staining. This sample was then analyzed using atomic force microscopy (AFM). The imaging revealed that rRNA molecules exhibit a multi-branched rod-like structure with high regularity. Based on their size and morphology, the rRNA molecules were classified into three distinct types, corresponding to 28S, 18S, and 5S rRNA, as predicted by computational models. All of them share similar secondary structures. The structural data obtained from 28S, 18S, 5.8S, and 5S rRNAs supports the thermodynamically stable secondary structures previously proposed. This study provides direct visual evidence of rRNA architecture at the single-molecule level, enhancing our understanding of ribosome structure and function.

Study Desk And Chair Set

Children's Study Desk and chair set is furniture for children aged 3-18 to learn and write homework. The table has a lot of storage space. The platen is made of solid wood core material. The table board is adjustable, and the table legs can be raised and lowered to meet various needs. A tall child can do homework and draw; the Study Chair is an ergonomic chair that can be adjusted to protect the child's spine. The seat cushion is made of latex, which is very comfortable and will not be tired, so that children can learn healthily and happily.study table and chair, the height of the table and chair can be adjusted according to the height of the child. In addition to the most basic "cushion height" that can be adjusted, the "armrest and back cushions" can also be adjusted according to the child's thigh length and waist height. Adjust so that the child does not sit in the wrong position due to the improper height of the table and chair. And the chair is designed according to children's ergonomics, unlike ordinary chairs that are straight up and down, there is a double-back curve design, which supports the child's back and ensures that the waist and hips fit perfectly with the chair.
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