Experimental principle of human neuronal apoptosis inhibitory protein (NAIP) ELISA Kit English name: Human neuronal apoptosis inhibitory protein, NAIP ELISA 6. Serum: Blood coagulates naturally at room temperature for 10-20 minutes, centrifuged for about 20 minutes (2000-3000 rpm). Collect the supernatant carefully and centrifuge again if a precipitate appears during storage. Avoid any cell stimulation during the operation. Use test tubes free of pyrogens and endotoxins. The ergonomic non-slip design and high quality plastic and TPR materials make the Bottle Opener or Jar Bottle Opener very easy to use and clean. Ideal for arthritic hands, seniors, anyone with limited hand strength, anyone with long fingernails, and anyone looking for a better, faster, easier way to open all of the sealed containers in their kitchen. Also this kitchen Can Opener are small and lightweight, just need small space, also can be hang on hooks for easy access. Bottle Opener Beer Bottle Opener,Can Opener,Bottle Opener,Metal Bottle Opener RealSheen Lifestyle Enterprise Limited , https://www.realsheen.com
1. The level of human neuronal apoptosis inhibitory protein (NAIP) ELISA Kit was measured by double antibody sandwich method.
2. First, coat the microplate with purified human neuronal apoptosis inhibitory protein (NAIP) ELISA Kit antibody to make a solid phase antibody.
3. Then add the Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit to the microwells coated with mAb in turn, and then combine with the HRP-labeled Neuronal Apoptosis Inhibitory Protein (NAIP) ELISA Kit antibody to form an antibody-antigen-enzyme The target antibody complex is washed with a substrate TMB for color development after thorough washing. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid.
4. The color depth is positively correlated with the neuronal apoptosis inhibitory protein (NAIP) ELISA Kit in the sample.
5. Finally, the absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of human neuronal apoptosis inhibitory protein (NAIP) ELISA Kit in the sample was calculated from the standard curve.
Objective: To determine the content of neuronal apoptosis inhibitory protein (NAIP) ELISA Kit in human serum, plasma and related fluid samples.
7. Plasma: EDTA or sodium citrate should be selected as the anticoagulant according to the requirements of the specimen. After mixing for 10-20 minutes, centrifuge for about 20 minutes (2000-3000 rpm) to remove particles. Collect the supernatant carefully, and centrifuge again if there is any precipitate.
safety:
1. Avoid direct contact with stop solution and substrate. If you accidentally come into contact with these liquids, please rinse them with water as soon as possible.
2. Do not eat, drink, smoke or use cosmetics during the experiment.
3. Do not touch any ingredients in the kit with your mouth.
4 Keep Elisa kit away from children's own items:
1. 37 ℃ incubator
2. Standard specification microplate reader
3. Precision pipette and disposable tip
4. Distilled water
5. Disposable test tube
6. Absorbent paper
Storage conditions and validity period:
1. Kit storage :; 2-8 ℃.
2. Validity: 6 months Product range:
Human, mouse, rat, pig, rabbit, other animal cytokines; apoptosis, active peptides, autoantibodies, thrombosis and hemostasis,
Bone metabolism, liver fibrosis, tumor, hormone endocrine, autoantibody research ELISA test kit The above articles are for reference only. More products please visit the website: