Precautions for using pipette Cautions for the use of pipettes: Kitchen Strainer ,Sink Strainer,Kitchen Sink Strainer,Basket Strainer NINGBO HOZ TRADING CO.,LTD , https://www.hoz-kitchenware.com
1. It is a normal adjustment method to set the pipetting volume from a large range to a small range. When the scale is turned counterclockwise to adjust from a small range to a large range, the scale should be adjusted to exceed the set volume scale, and then adjusted back to the set volume. This will ensure the accuracy of the pipette
2. Assemble the pipette tip. Insert the pipette tip vertically into the pipette tip, turn left and right half a turn, and tighten. The method of hitting the pipette tip with a pipette is very undesirable. This operation for a long time will cause the parts of the pipette to loosen due to the impact, which will seriously cause the knob to adjust the scale to be stuck.
3. Liquid suction and liquid discharge The tip of the vertical suction pipette tip is immersed below 3mm in the liquid surface. The pipette head is pre-wetted in the liquid before the liquid is sucked. When the liquid is sucked slowly and the liquid is discharged slowly, if the amount is small, the tip of the pipette tip should be reliable.
4. Pipettes that have aspirated liquid should not lie flat. The liquid in the gun head can easily contaminate the inside of the gun and may cause the spring of the gun to rust
5. After each experiment, the pipette gun should be adjusted to the maximum scale to allow the spring to return to the original model to extend the life of the pipette gun.
6. When sucking liquid, be sure to release your thumb slowly and steadily. Never let go suddenly, to prevent the solution from being drawn too fast and rushing into the pipette to corrode the plunger and cause air leakage.
7. In order to obtain higher precision, the tip needs to draw the sample solution in advance, and then formally pipet, because when the serum protein solution or organic solvent is drawn, a layer of "liquid film" will remain on the inner wall of the tip, resulting in a discharge volume. Too small to produce errors.
8. Liquids with high concentration and viscosity will produce errors. The compensation amount to eliminate the error can be determined by experiment. The compensation amount can be set by changing the reading of the reading window with the adjustment knob.
9. You can use the analytical balance to weigh the weight of pure water and calculate it to correct the liquid picker. 1mL of distilled water weighs 0.9982g at 20 ℃
10 When setting the range, please pay attention to? Rotate to the desired range? The numbers are clearly displayed in the display window. The set range is within the range of the pipette. Pipette.
11. Pipettes are strictly forbidden to absorb highly volatile and corrosive liquids (such as concentrated acid, concentrated alkali, organic matter, etc.).
12. It is strictly forbidden to pipette and mix the liquid.
13. Do not use a large range of pipettes to remove small volumes of liquid, so as not to affect the accuracy. At the same time, if you need to remove a large amount of liquid outside the range, please use a pipette for operation.