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What to pay attention to when purchasing a gene amplification PCR instrument
Shanghai Xinzhuang Instrument Co., Ltd. offers the Xinzhuang (LNB) PCR system, a powerful tool for large-scale in vitro DNA amplification using DNA polymerase. This technique enables specific replication of genetic material by leveraging the enzymatic activity of DNA polymerase. Modern PCR technology can amplify a target gene up to 10 billion to 100 billion times, making it an essential method in molecular biology and diagnostic testing.
PCR instruments are typically categorized into four types based on their application and detection requirements: standard PCR machines, gradient PCR instruments, in situ PCR systems, and real-time fluorescence quantitative PCR devices. Each type is designed to meet different experimental needs, from basic amplification to precise quantification.
The PCR process involves three fundamental steps: denaturation, annealing, and extension. First, the template DNA is heated to 90–95°C to separate the double-stranded DNA into single strands. Next, the temperature is lowered to 55–60°C, allowing primers to bind to the complementary sequences on the single-stranded DNA. Finally, at around 70–75°C, DNA polymerase extends the primers, synthesizing new DNA strands using dNTPs as building blocks. This cycle repeats, doubling the amount of target DNA with each iteration. A typical PCR run can complete millions of copies within 2 to 3 hours, depending on the protocol and instrument used.
Shanghai Xinzhuang’s PCR instruments are known for their fast heating rates, accurate temperature uniformity, and a wide range of models tailored for different laboratory settings. Whether you're conducting routine experiments or advanced research, our equipment ensures reliable performance and precision.
Our company specializes in producing high-quality laboratory instruments, combining cutting-edge technology with rigorous quality control. We welcome your inquiries and feedback to help improve our products and services. For more information, visit our website or contact us directly.
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