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What to pay attention to when purchasing a gene amplification PCR instrument
Shanghai Xinzhuang Instrument Co., Ltd. offers the Xinzhuang (LNB) PCR system, a powerful tool for large-scale in vitro DNA amplification using DNA polymerase. This technique enables specific DNA replication by leveraging the enzymatic activity of DNA polymerase. Modern PCR technology can amplify a target gene up to 10 billion to 100 billion times, making it an essential method in molecular biology, diagnostics, and genetic research.
PCR instruments are typically categorized into four main types based on their function and application: standard PCR machines, gradient PCR instruments, in situ PCR systems, and real-time fluorescence quantitative PCR devices. Each type is designed to meet different experimental needs, from basic amplification to advanced quantification and analysis.
The PCR process involves three fundamental steps: denaturation, annealing, and extension. First, the template DNA is heated to around 90–95°C to separate the double-stranded DNA into single strands, allowing primers to bind. Next, the temperature is lowered to 55–60°C, enabling the primers to hybridize with their complementary sequences on the single-stranded DNA. Finally, at around 70–75°C, DNA polymerase extends the primers by adding nucleotides in a sequence-specific manner, creating a new DNA strand that serves as a template for the next cycle. Each cycle takes approximately 2–4 minutes, and after 2–3 hours, millions of copies of the target gene can be produced.
Shanghai Xinzhuang’s PCR instruments are known for their fast heating rates, precise temperature uniformity, and reliable performance. With a range of models available, they cater to various laboratory requirements. Whether you're conducting basic research or advanced diagnostics, our equipment ensures accuracy and efficiency.
If you're looking for a high-quality PCR instrument, Shanghai Xinzhuang is your trusted partner. Our professional team is ready to provide expert guidance and support. For more information, feel free to contact us. We also welcome your feedback and suggestions to help us improve our products and services.
For tips on choosing the right PCR instrument, check out our detailed guide here.