New cell culture method with high efficiency and short cycle

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A research team in Japan recently reported that they have developed a tiny bead for cell culture, which not only facilitates cell attachment, but also reduces the temperature to separate cells and beads as quickly as possible, which helps speed up experiments and research schedule.

The research team led by Professor Okano Hiroo of Tokyo Women ’s Medical University said that they used polystyrene to make beads with a diameter of 0.1 mm to 0.2 mm, and then coated the surface with a temperature-sensitive polymer material. After stringing such a large number of beads into a flask containing the culture solution, the beads will float on the surface of the liquid. By rotating the flask, the beads can fully contact the culture solution.

Tests show that under conditions above 32 degrees Celsius, hamster ovary cells in the culture medium are very easy to attach to the beaded polymer coating. If you want these ovarian cells to get rid of the beads after maturation, you only need to reduce the temperature of the culture environment to below 20 degrees Celsius. About two hours later, more than 90% of the ovarian cells will fall off the beads.

Okano Kofu pointed out that after culturing the cells by the above method for a week to 11 days, the cultured cells can be smoothly detached from the beads by reducing the temperature. If the beaded beads are made smaller, the cells will attach more easily to their surface, but it is difficult to separate them by cooling.

Mr. Okano said that when cultivating cells using a general petri dish, although enzymes can be used to detach the cells from the inner bottom surface of the petri dish, it will cause some of the proteins in the cells to decompose. After using the "bead culture method", the cells can be completely shed. In addition, since a large number of beads on the string of beads are much larger than the surface area of ​​the bottom surface of the petri dish, more cells can be cultured.

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