CRISPR equipped with phage allows "super bacteria" to commit suicide!

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It is well known that the CRISPR system is originally an immune means for bacteria to resist external virus infection, but perhaps one day in the future, CRISPR technology can help people kill the bacteria themselves. By transforming phage to carry CRISPR operating elements, scientists hope that the tool will effectively kill resistant bacteria and can be used to transform the body's microbiome.

CRISPR equipped with phage allows "super bacteria" to commit suicide!

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The full name of CRISPR is "Clustered regular interspaced short palindromic repeats", which is a repeated palindrome sequence. Bacteria use the CRISPR system to resist phage infection. At the time of the initial infection, the infected bacteria are able to integrate their CRISPR sequences into the interior of the phage. In subsequent infections, CAS enzymes in bacteria can quickly detect and shear them.

Twenty-five years ago, researchers realized that this system could be used for genetic engineering, and a new genetic engineering tool was born. The technology has developed rapidly in recent years and has also shown great value in the treatment of HIV infection. Although it can't be said that there is no risk, the CRISPR technology has indeed undergone some changes. It has been a magical thing to take it out of the original bacterial genome and use it to kill bacteria.

The use of bacteriophage killing bacteria is not a new concept, and the related clinical treatment can be traced back to 1920. This method is very interesting because its specificity is much higher than that of antibiotics: it only kills specific target bacteria. In addition, the virus can penetrate the sticky outer membrane of the bacteria, thereby increasing its killing level.

At present, many research institutions and biotech companies are constantly challenging the boundaries of CRISPR technology, including designing CRISPR sequences that specifically recognize antibiotic resistance genes, thereby effectively killing resistant bacteria.

In recent years, our understanding of the complexity of microorganisms and the environment has gradually deepened. In addition to the seriousness of bacterial resistance, the diversity of intestinal microbes has also deeply affected the occurrence of many human diseases. Therefore, advances in this technology may help us improve the effectiveness of targeting killing bacteria.

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