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Different culture media need different sterilization methods according to their performance.
The type of medium can be categorized based on four main factors: composition, physical state, intended use, and the specific microbial species it supports. Sterilization is a crucial step in preparing culture media, and different types of media may require varying sterilization techniques depending on their properties. Here’s what Shanghai Hengyuan Bio Company recommends:
1. **Common Sterilization Methods**: Most media are typically sterilized using high-pressure moist heat at 121°C for 15 minutes. However, some specialized media might require alternative conditions, such as 115°C for 20 minutes, as specified by the user or manufacturer.
2. **Boiling Sterilization**: Certain media, especially those that are less sensitive to heat, can be sterilized by simply boiling them. This method is often used when more advanced equipment is not available.
3. **Filtration Sterilization**: For heat-sensitive media or substances that could degrade under high temperatures, membrane filtration is the preferred method. It ensures sterility without altering the chemical integrity of the medium.
4. **Ready-to-Use Reagents**: Some reagents come pre-sterilized and ready for immediate use. These should be handled according to international standards or the supplier’s instructions, without additional sterilization steps.
**Disinfection and Sterilization of Equipment**:
- **Wet Heat Sterilization**: This is commonly performed using an autoclave. Glassware, pipette tips, and plastic containers are typically sterilized at 121°C for 20 minutes. The effectiveness of the autoclave should be regularly tested according to GB 15981 standards.
- **Dry Heat Sterilization**: Used for items like glassware and stainless steel tools, this method involves heating at 160°C for 2 hours or 180°C for 1 hour. It is ideal for materials that cannot withstand moisture.
**Additional Sterilization Practices**:
- Metal instruments should first be wiped with alcohol, dried, and then sterilized. They can also be flame-sterilized before use.
- Plastic items can often be sterilized using high-temperature methods, while organic solvents are better suited for filtration.
- Hands and work surfaces should be cleaned with alcohol, and the lab bench and room should be exposed to UV light for additional disinfection.
- Culture media must be sterilized using moist heat, but the exact conditions vary depending on the type of media. For example, plant tissue culture media differ from those used for microbial cultures.
For more detailed information and expert guidance, feel free to visit the official website of Shanghai Hengyuan Bio Company. They offer a wide range of high-quality products, including complete media, ELISA kits, antibodies, serum, standards, and other essential biological reagents. Stay updated with the latest in experimental techniques and scientific advancements!