Determination of Protein in Tea by Kjeldahl Method

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The taste of the tea is slightly bitter, sweet and cool. Can clear the head, wake up the spirit, solve the polydipsia, benefit urination, eliminate food, detoxification. Used for wind and heat, dizziness and dizziness; heat and polydipsia, or excessive drinking; more sleep, sleep, tired and tired; urinary short red is not good, or edema and oliguria; greasy food, indigestion; hot and humid diarrhea, dysentery.

The role of tea: diuretic detoxification, degreasing, promoting digestion, eliminating fatigue, enhancing memory, anti-inflammatory and antibacterial, prevention of dental caries, anti-cancer, anti-radiation, anti-aging, prevention of high blood pressure.

Ingredients in tea: protein, amino acids, alkaloids, tea polyphenols, sugars, lipids, vitamins, mineral elements.

Determination of Protein in Tea by Kjeldahl Method

Reagents and main instruments

1. Concentrated sulfuric acid (AR)

2, potassium sulfate (AR)

3. Copper sulfate (AR)

4, 3.0% NaOH solution

5, 2% boric acid solution

6, 0.05mol / L standard HCL solution

7. 0.1% mixed indicator: 0.1% methyl red alcohol solution and 0.1% methyl blue alcohol solution, mixed according to 1:1. Shanghai Chuangsai provides a methyl red indicator kit, product number: C015217-5ml*2, the price is 20 yuan.

8, micro-Kelvin ammonia meter

Processing sample

Accurately weigh 2g of sample, carefully transferred into the flask, and then add CuSO4 1g, K2SO4 10g and concentrated H2S04 50mL. Heat it in an electric furnace until the liquid turns blue-green transparent, then cool and dilute to 100mL. Shanghai Chuangsai provides 2-bromobenzeneboronic acid (containing an amount of anhydride), 244205-40-1, commodity number: C12-B2889-5G, price 740.6 yuan.

Determination

After washing the micro-Kjeldahl ammonia unit with steam, accurately remove 10.00mL of chemical solution, measure 25mL of NaOH, add to the reaction chamber, steam distillate to boric acid solution from grape purple to green, and finally use 0.05mol/L The HCL solution was titrated to pale grape purple.

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