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Rat atrial natriuretic peptide (ANP) elisa technology kit
**Rat Atrial Natriuretic Peptide (ANP) ELISA Kit – High-Quality Domestic Supplier**
*Guangrui Bio – Trusted Source for Reliable Research Reagents*
This ELISA kit is intended for research purposes only and is designed to quantify the levels of Atrial Natriuretic Peptide (ANP) in rat serum, plasma, and tissue-related fluids. The method employed is a double-antibody sandwich ELISA, which ensures high specificity and sensitivity.
The procedure involves coating a microplate with purified anti-ANP antibodies. After adding the sample, HRP-conjugated ANP antibodies bind to the captured ANP, forming an antibody-antigen-enzyme complex. Following washing steps, TMB substrate is added, leading to a color change that correlates with the concentration of ANP in the sample. The reaction is stopped with a stop solution, and absorbance is measured at 450 nm using a microplate reader. A standard curve is then used to calculate the exact ANP concentration in the samples.
**Kit Components:**
- 48-well or 96-well configurations available
- Coated microplate with anti-ANP antibodies
- Standard solutions (540 ng/L)
- Enzyme-labeled reagent
- Sample diluent, wash buffer, TMB substrates (A and B), and stop solution
- Storage instructions: 2–8°C
**Sample Preparation Guidelines:**
- **Serum/Plasma:** Centrifuge blood samples at 2000–3000 rpm for 20 minutes.
- **Urine/Cerebrospinal Fluid:** Centrifuge at the same speed and collect supernatant.
- **Cell Culture Supernatant:** Centrifuge after collection; for intracellular components, lyse cells via freeze-thaw cycles.
- **Tissue Homogenate:** Homogenize in PBS, centrifuge, and collect the supernatant.
**Important Notes:**
- Avoid repeated freezing and thawing of samples.
- Do not use samples containing NaN3, as it inhibits HRP activity.
- Always prepare a standard curve and perform duplicate measurements for accuracy.
- Use fresh reagents and avoid cross-contamination by using separate pipettes for each step.
- Store all components at 2–8°C and use within 6 months from the date of receipt.
**Performance Characteristics:**
- Linear regression coefficient (R²) ≥ 0.990
- Intra-batch and inter-batch variation < 9% and < 11%, respectively
- Detection range: 20 ng/L – 400 ng/L
This ELISA kit offers a reliable and efficient method for quantifying ANP levels in various biological samples, making it ideal for researchers studying cardiovascular function, fluid balance, and related physiological processes. Always follow the manufacturer’s instructions carefully to ensure accurate and reproducible results.