Different ELISA kits for each sample

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The percentage of positive samples in this study was determined by the NAT or RIBA response. ELISA kits In this study, among the observed reaction kits, 16 (30.19%) of 53 samples were positive, and 6 of NAT (16.22%) were negative. But RIBA is positive. In this group of tests, 53 (63.1%) positive NAT and 23 RIBA negative RIBA showed positive. The nature of the NAT and RIBA samples was positive (53.8%). One of the reasons for the ELISA kit may be embodied in one and two ELISA kits. The research team used the same experimenter's research samples as reference. The probability of a positive sample reaction is likely to be higher than the reaction of the sample in the two kits. In Hungary, in a study done by Hejjas et al., 32 out of 11 samples in blood donation (34.37%), which were reacted in harmony in 5 ELISA kits, the positive expression of hepatitis C virus RNA was PCR. These results coincide with this study (30.19% positive NAT).

From the above studies, it can be concluded that ELISA kit single ELISA kit reaction samples have a high probability of being shown by negative NAT or RIBA. The samples are reflected by two different ELISA tests for anti-HCV, and the other probabilities of the ELISA kit are confirmed by NAT or RIBA multiple response data. The reason may be that the two sequences use high positive predictive values, because the sample reaction of these two detection methods has a higher probability of being true. Not just those in one reaction. Even if it is mentioned in the WHO guidelines, if a confirmatory test is not available, a backup measurement can be used, which is used as the main measurement to confirm the status of the sample.

Anti-HCV enzyme-linked immunosorbent reaction of blood donors, ELISA kits but negative NAT and RIBA are not necessarily permanently delayed. They may re-accept these donors to make a huge contribution, as it allows regular valuable motivational donors whose blood has been proven safe. The recipient's blood product was positive from the previous anti-HCV ELISA, the ELISA kit gene-PCR was negative, RIBA was undefined or negative donor donation, and there was no hepatitis C virus infection. These donors have not been infected. The author has suggested that these donors can re-enter and provide donations, and future donations will be negative for anti-HCV ELISA.

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