Experimental principle of rat Î² amyloid protein (Î²AP) Elisa kit This kit uses the double antibody sandwich method to determine the level of rat Î² amyloid protein (Î²AP) in the specimen. Microporous plates were coated with purified rat Î²-amyloid (Î²AP) antibody to make solid-phase antibodies, and Î²-amyloid (Î²AP) was added to the monoclonal antibody-coated microwells in turn, followed by HRP-labeled Î²-amyloid The antibody like protein (Î²AP) binds to form an antibody-antigen-enzyme-labeled antibody complex. After thorough washing, the substrate TMB is added for color development. TMB is converted into blue under the catalysis of HRP enzyme, and into the final yellow under the action of acid. The color depth is positively correlated with the Î²-amyloid protein (Î²AP) in the sample. The absorbance (OD value) was measured with a microplate reader at a wavelength of 450 nm, and the concentration of rat amyloid Î² (Î²AP) in the sample was calculated by a standard curve.
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